Sunday, July 13, 2014

P. larvae LC50 Pilot

Checked honey bee larva via microscopy. It appears that the larva that was alive yesterday is still alive today. It was difficult to determine if they truly were still alive. The didn't appear to be degraded (only a circulatory system present) like the others that were definitely dead the second day. However, they didn't ungulate as the confirmed alive did either. Perhaps they are already dead, but preserved in the large amounts of BAD present.

The surviving larva were fed an additional 30 uL BAD (pipetted on top of them). This makes 9 surviving larva. I will continue to monitor their survival by microscopy as well as comparing the sizes of tomorrow to today. I will also make note if any of their BAD food has decreased from their wells because they have been eating it. They surviving larva do appear to be a bit larger than those that had died already.

//EWW

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